IIT JAM Biotechnology 2023 Question Paper with Answer Key

Which one of the following compounds inhibits the polymerization of tubulin to microtubules in animal cells?

1. AATP
2. BTaxol
3. CThymosin
4. DVinblastine

Arrange the following elements in increasing order of their electronegativity according to the Pauling scale

C, Na, Be and Br

1. ABe, Na, C, Br
2. BBr, C, Na, Be
3. CNa, Be, C, Br
4. DNa, C, Be, Br

Which one of the following is NOT a plant vascular tissue?

1. APhloem
2. BPeriderm
3. CStele
4. DXylem

A growing shoot of a germinating seedling encounters an underground obstacle. Which one of the following hormones elicits ‘triple response’ to the underground obstacle?

1. AAuxin
2. BCytokinin
3. CEthylene
4. DGibberellins

Which one of the following pairs of antibodies contains ‘J-chain’ in their multimeric form?

1. AIgA and IgE
2. BIgA and IgM
3. CIgD and IgE
4. DIgD and IgG

Restriction enzymes that recognize the same nucleotide sequence but cleave at different positions are called

1. Aheterohypekomers
2. Bisocaudomers
3. Cisoschizomers
4. Dneoschizomers

Which one of the following does NOT belong to the freshwater ecosystem?

1. AEstuary
2. BLentic
3. CLotic
4. DWetland

Which one of the following is transcribed by RNA polymerase III in eukaryotes?

1. A18S rRNA
2. B28S rRNA
3. CmiRNA
4. DtRNA

Given the following sets:

A = {2, 4, 6, 8, 10, 12}B = {8, 10, 12, 14, 16, 18}C = {7, 8, 9, 10 11, 12, 13}

(A ∩ B) ∪ (B ∩ C) is

1. A{8, 10, 12, 14}
2. B{8, 10, 12}
3. C{7, 8, 10, 11, 12, 13, 14}
4. D{4, 6, 7, 8 10, 11, 12, 13}

Rain is falling vertically with a speed of 40 m s⁻¹. Wind starts blowing with a speed of 16 m s⁻¹ in the west to east direction. How should a person, who is standing, hold his umbrella to avoid getting wet?

1. AAt an angle of about 22° with vertical towards east
2. BAt an angle of about 22° with vertical towards west
3. CAt an angle of about 66° with vertical towards east
4. DAt an angle of about 66° with vertical towards west

Which one of the following statements about the G1 checkpoint of eukaryotic cell division cycle is INCORRECT?

1. ACell assures the existence of favorable extracellular environment
2. BCell assures the DNA has no damage
3. CCell assures the damaged DNAs are directed for repair mechanism
4. DCell assures complete replication of DNA

Determine the correctness or otherwise of the following Assertion [a] and the Reason [r].

Assertion [a]: Nitric oxide is involved in transient paracrine and autocrine signaling.

Reason [r]: Nitric oxide is highly reactive, with a lifetime of few seconds, yet can diffuse freely across membranes

1. ABoth [a] and [r] are true and [r] is the correct reason for [a]
2. BBoth [a] and [r] are true but [r] is not the correct reason for [a]
3. CBoth [a] and [r] are false
4. DOnly [a] is true but [r] is false

In mice, a trait is determined by a dominant allele Y and recessive allele y. What proportion of the offspring from a YY × yy cross is expected to be homozygous recessive in F1 generation?

1. A0
2. B0.25
3. C0.5
4. D1

Match the molecules in Group I with the type of bonds present in them, in Group II

1. AP-4, Q-1, R-3, S-2
2. BP-2, Q-3, R-1, S-4
3. CP-4, Q-3, R-1, S-2
4. DP-4, Q-3, R-2, S-1

What is the splitting pattern of proton H^a of the following compound in its ¹H NMR spectrum?

1. ADoublet
2. BDoublet of doublet
3. CMultiplet
4. DTriplet

Which one of the following statements is correct about solute transport across membranes?

1. APassive transporters decrease the activation energy and does not facilitate the transport of polar compounds
2. BThe direction in which a charged solute tends to move spontaneously across a membrane does not depend on the electrical gradient across the membrane
3. CAll ABC transporters do not have nucleotide binding domain
4. DP-type ATPases get reversibly phosphorylated as a part of transport cycle

Match the type of DNA repair mechanism in Group I with the enzyme(s) involved in Group II

1. AP-4, Q-1, R-3, S-2
2. BP-4, Q-2, R-1, S-3
3. CP-4, Q-1, R-2, S-3
4. DP-2, Q-1, R-4, S-3

Tetracycline binds to the

1. A30S subunit and inhibits aminoacyl-tRNA binding
2. B50S subunit and inhibits aminoacyl-tRNA binding
3. C30S subunit and prevents codon:anticodon interactions
4. D50S subunit and blocks exit of growing polypeptide chain

In the ‘Southern blot’ technique, which of the following reagents is used to detect the presence of a desired DNA fragment?

1. AEthidium bromide
2. BDNA probe
3. CSilver nitrate
4. DDNase

The pedigree given below shows individuals affected (shaded circles/rectangles) by chronic hypertension. Assuming 100% penetrance, the inheritance of this trait is

1. Aautosomal dominant
2. Bautosomal recessive
3. Csex-linked dominant
4. Dsex-linked recessive

Which one of the following statements about photoproteins in plants is INCORRECT?

1. APhytochromes are activated by red light
2. BPhytochromes are inactivated by far-red light
3. CCryptochromes are sensitive to blue light
4. DPhototropins are insensitive to blue light

Match the microorganisms in Group I with the human disease in Group II

1. AP-4, Q-3, R-1, S-2
2. BP-1, Q-2, R-4, S-3
3. CP-3, Q-1, R-2, S-4
4. DP-2, Q-1, R-3, S-4

Which one of the following is correct in the case of conjugation of a high frequency recombination (Hfr) strain with F⁻ strain of E. coli?

1. ARecombination frequency is high, F factor transfer frequency is low
2. BRecombination frequency is high, F factor transfer frequency is high
3. CRecombination frequency is low, F factor transfer frequency is high
4. DRecombination frequency is low, F factor transfer frequency is low

Determine the correctness or otherwise of the following Assertion [a] and the Reason [r].

Assertion [a]: The cardiovascular organization called double circulation provides vigorous flow of blood to the brain, muscles, and other organs.

Reason [r]: The blood is pumped a second time after it loses pressure in the capillary beds of the lungs or skin.

1. ABoth [a] and [r] are true and [r] is the correct reason for [a]
2. BBoth [a] and [r] are true but [r] is not the correct reason for [a]
3. CBoth [a] and [r] are false
4. D[a] is true but [r] is false

The inability in humans to taste capsaicin resides in a single gene difference between two alleles P and p. The allele P for tasting is dominant over the nontasting allele. In a population of 400 individuals in Hardy-Weinberg equilibrium, 64 are nontasters. How many individuals are heterozygous for the gene?

1. A64
2. B128
3. C144
4. D192

A genetic linkage map represents the

1. Arelative locations of genes on a chromosome
2. Bdistribution of the mutational hotspots
3. Cphylogenetic linkage among organisms
4. Daccurate physical distances among loci

Class II MHC molecules are NOT expressed by

1. AB-cells
2. Bdendritic cells
3. Cmacrophages
4. DT-cells

Which one of the following enzymes is required to ensure the replication of a negative-sense or negative-strand RNA virus?

1. ADNA-dependent RNA polymerase
2. BDNA polymerase
3. CRNA-dependent DNA polymerase
4. DRNA-dependent RNA polymerase

The relationship between birth weight and degree of survival (log [survivors / non-survivors]) in 6908 human births in an obstetric hospital is shown in the figure below. The mode of selection for birth weight is

1. Adirectional
2. Bdisruptive
3. Cdiversifying
4. Dstabilizing

Match the recombinant DNA products in Group I with its application in Group II

1. AP-1, Q-2, R-3, S-4
2. BP-1, Q-3, R-4, S-2
3. CP-3, Q-1, R-4, S-2
4. DP-4, Q-3, R-1, S-2

Which of the following statement(s) is/are correct about telophase?

1. ADaughter chromosomes are yet to form
2. BNew nuclear envelop starts to reassemble
3. CDivision of cytoplasm begins
4. DNuclear membrane disappears

The characteristic morphological change(s) in cells undergoing apoptosis is/are

1. Aformation of blebs on cell surface
2. Bswelling and bursting of cells
3. Ccollapse of the cytoskeleton
4. Dcondensation and fragmentation of nuclear chromatin

A species of fish living in a lake are separated by drying up of the lake into two separate lakes. After several hundreds of years of separation, the two groups are unable to mate. These groups are now considered to be different _______.

1. Acommunities
2. Borganisms
3. Cpopulations
4. Dspecies

Which of the following compound(s) is/are aromatic?

1. A
2. B
3. C
4. D

Which of the following statement(s) is/are correct for the following compound?

1. AIt can have a maximum of four stereoisomers
2. BIt can have a maximum of two stereoisomers
3. CIt is a chiral compound
4. DIt is an achiral compound

Which of the following is/are essential feature(s) of high-fidelity DNA polymerases used in polymerase chain reaction?

1. A5′→3′ exonuclease activity
2. BEndonuclease activity
3. C3′→5′ exonuclease activity
4. DOptimum temperature for activity ≥72 °C

Which of the following option(s) represent(s) the evolutionary relationship between the bird and bat wings as structures for flying?

1. Aanalogous
2. Bconvergence
3. Cdivergence
4. Dhomologous

Different segments of a photosynthetic filamentous alga are exposed to different wavelengths of light as shown below. After a period of time, bacteria known to migrate towards high oxygen concentration, is spread on the surface of the alga. Which region(s) of the alga will have maximum bacterial congregation?

1. AP and R
2. BP and Q
3. COnly P
4. DOnly Q

Hyperventilation (breathing rapidly and deeply) causes which of the following event(s) in the arterial blood?

1. ADecrease in CO₂ concentration
2. BDecrease in proton concentration
3. CIncrease in pH
4. DIncrease in O₂ concentration

Which of the given statement(s) about synthetic oligonucleotides is/are correct?

1. AChemical synthesis extends the DNA chain from 3′→5′ end
2. BThey can be utilized for site-directed mutagenesis
3. CChemical synthesis extends the DNA chain from 5′→3′ end
4. DThey can be utilized as radiolabeled probes

The net number of molecule(s) of NADH formed from one molecule of glucose in glycolysis under aerobic conditions is/are _______.

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The number of possible unique combination(s) of linear tetrapeptides that can be made from four different amino acids using each amino acid only once in the chain is/are _______.

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Among i-BuNH₂, NH₃, Me₂NH, EtNH₂, the number of compound(s) more basic than MeNH₂ is/are _______.

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Among K⁺, Li⁺, Rb⁺, Cs⁺, the number of cation(s) having ionic radii more than Na⁺ is/are _______.

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Among the five fragments given below,

the number of fragment(s) accelerated to the analyzer tube in mass spectrometer with electron ionization is/are _______.

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A restriction endonuclease has a recognition site of 3 bases. Assuming random arrangement of nucleotides, the probability that this endonuclease will cut a piece of DNA is _______ (rounded off to three decimal places).

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A massless ideal spring is hanging vertically. A sphere of mass of 500 g, suspended from the spring, stretches the spring from its initial position by 50 cm when it reaches equilibrium. The force constant of the spring is _______ N m⁻¹. (Use g = 10 m s⁻²)

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Whales can dive undersea to depths of 2 km. The pressure on the whale at this depth (ignoring atmospheric pressure) is _______ × 10⁶ Pa. (Density of sea water = 1 g cm⁻³ and g = 10 m s⁻²)

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The order of differential equation d³y/dx³ + 2 d²y/dx² − 3 dy/dx + 6x⁴y = 0 is _______.

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The value of lim_x→−3 (2x + 6)/(x + 3) is _______.

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The ΔG′ and K′_eq values of ATP hydrolysis are −32.34 kJ mol⁻¹ and 4.6 × 10⁵, respectively. The ΔG′ and K′_eq values of enzymatic hydrolysis of glucose-6-phosphate to glucose and phosphate are −13.18 kJ mol⁻¹ and 203.8, respectively. The ΔG′ value of reaction of glucose-6-phosphate formation from glucose and ATP by hexokinase is _______ kJ mol⁻¹ (rounded off to 2 decimal places). [All reactions are carried out at pH 7.0 and 25 °C].

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K_m and V_max of an enzyme preparation are 5 µM and 30 µM min⁻¹ respectively. Considering, K_i value of competitive inhibitor is 60 µM, the velocity (V₀) of this enzyme-catalyzed reaction in the presence of 200 µM of substrate and 600 µM of competitive inhibitor is _______ µM min⁻¹ (rounded off to two decimal places).

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The heat required to convert 2 kg of water at 20 °C in a calorimeter to steam at 100 °C and at atmospheric pressure (1 atm) is _______ kJ. (Specific heat capacity of water is 4.2 kJ kg⁻¹ K⁻¹ and latent heat of steam is 2256 kJ kg⁻¹)

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An electron is accelerated from rest through a potential difference of 200 V. The de Broglie wavelength associated with this electron is _______ nm. (Rounded off to 2 decimal places) (Planck’s constant = 6.6 × 10⁻³⁴ J s, 1 eV = 1.6 × 10⁻¹⁹ J, mass of an electron = 9.1 × 10⁻³¹ kg)

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Given data consists of distinct values of x_i occurring with frequencies f_i. The mean value for the data is _______. (rounded off to one decimal place)

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A random variable X and its probability distribution is given below. The value of P(X < 5) is _______. (rounded off to one decimal place)

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A protein solution of 1 µM has transmission of 40 % at 280 nm, when measured in a 1 cm cuvette using a UV-Visible spectrophotometer. The transmission of the same solution, when measured using a 2 cm cuvette is _______ %. (rounded off to the nearest integer)

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If a bacterial culture with a doubling time of 30 minutes starts with two cells, then the number of cells after 4 hours are _______.

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The rate of transcription in a bacterium is 50 nucleotides/min and the average molecular weight of an amino acid is 110 Da. Time taken for synthesis of the mRNA of a protein with molecular weight of 110 kDa is _______ min. (rounded off to one decimal place)

Assume no abortive transcriptions and no sequences upstream of the start codon.

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Consider a first order reaction A → B. The initial concentration of A is 100 mol L⁻¹ and the value of first order rate constant is 0.01 min⁻¹. The concentration of A after 10 min of reaction is __________ mol L⁻¹ (rounded off to one decimal place).

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