
All 60 questions from the IIT JAM 2024 Biotechnology (BT) paper, typed out in full, with the answer key and every diagram: Section A (MCQ), Section B (MSQ) and Section C (numerical answer).
IIT JAM 2024 Biotechnology was organised by IIT Madras. The paper has three sections by question type and four subjects: 43 Biology, 7 Chemistry, 5 Physics and 5 Mathematics questions. Every question is below in the order it was set, tagged with its topic. Tap Show answer under any question to check the answer.
Marking scheme
| Section | Questions | Marks each | Wrong answer | Max marks |
|---|---|---|---|---|
| A: MCQ (one correct option) | Q1–30 (10 × 1 mark, 20 × 2 marks) | 1 or 2 | −1/3 or −2/3 | 50 |
| B: MSQ (one or more correct) | Q31–40 | 2 | No negative marking | 20 |
| C: Numerical answer | Q41–60 (10 × 1 mark, 10 × 2 marks) | 1 or 2 | No negative marking | 30 |
| Total | 60 | 100 |
Which topics this paper tested
We tagged every question with its subject and topic. Biology carried 43 of the 60 questions; within it, Molecular Biology and Methods in Biology had the most. The charts below cover all four subjects.
| Subject | Questions | Marks |
|---|---|---|
| Biology | 43 | 72 |
| Chemistry | 7 | 12 |
| Physics | 5 | 8 |
| Mathematics | 5 | 8 |
| Total | 60 | 100 |
Biology
Chemistry
Physics
Mathematics
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Section A: Multiple choice (one correct option) (Q1–30)
30 questions (10 of 1 mark, 20 of 2 marks). A wrong answer loses 1/3 or 2/3 of the marks.
Which one of the following is a simple tissue system in plants?
- AEpidermis
- BParenchyma
- CPhloem
- DXylem
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In DNA replication, the Okazaki fragments are joined by
- ADNA helicase
- BDNA ligase
- CDNA polymerase
- DDNA primase
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The most abundant type of RNA in a metabolically active mammalian cell is
- AmRNA
- BrRNA
- CsnoRNA
- DtRNA
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Which organelle in a eukaryotic cell is the site of electron transport chain?
- AEndoplasmic reticulum
- BGolgi apparatus
- CMitochondrion
- DPeroxisome
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RNA is a polymer of
- Aglycosides
- Bribonucleosides
- Cribonucleotides
- Driboses
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Which one of the following is present in a bacterial cell?
- A28S rRNA
- B70S ribosome
- CChitinous cell wall
- DHistones
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Which color of light excites a natural GFP to emit green fluorescence?
- ABlue
- BGreen
- CInfrared
- DRed
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Which one of the following hormones promotes fruit ripening?
- AAbscisic acid
- BAuxin
- CEthylene
- DGibberellin
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Which one of the following has a catalytic RNA?
- ARibonuclease H
- BRibozyme
- CRNA polymerase I
- DRNA polymerase II
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The number of significant figures in a reported measurement of 0.00361 is
- A3
- B4
- C5
- D6
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Match the terminology in Group I with the stimulus in Group II that generates growth response of plants
| Group I | Group II |
|---|---|
| P. Gravitropism | 1. Light |
| Q. Phototropism | 2. Touch |
| R. Thigmotropism | 3. Chemical |
| S. Chemotropism | 4. Gravity |
- AP – 3, Q – 4, R – 2, S – 1
- BP – 2, Q – 1, R – 3, S – 4
- CP – 4, Q – 1, R – 2, S – 3
- DP – 4, Q – 2, R – 1, S – 3
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The correct hierarchy of taxa in the Linnaean classification of eukaryotes is
- Akingdom, class, phylum, order, family, genus
- Bkingdom, order, class, phylum, family, genus
- Ckingdom, phylum, order, family, class, genus
- Dkingdom, phylum, class, order, family, genus
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Which one of the following statements about polyploidy is correct?
- AAutopolyploids are derived from a single species
- BAutopolyploids are derived from two different species
- CAllopolyploids are derived from a single species
- DAllopolyploids are not fertile when mated with each other
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Which one of the following hormones is a tyrosine derivative?
- AEpinephrine
- BEstradiol
- CProgesterone
- DTestosterone
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Which one of the following immunoglobulins crosses the human placenta?
- AIgA
- BIgE
- CIgG
- DIgM
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Determine the correctness or otherwise of the following Assertion [a] and the Reason [r].
Assertion [a]: The resolving power of a transmission electron microscope is higher than that of the light microscope.
Reason [r]: The wavelength of electrons is shorter than that of visible light.
- ABoth [a] and [r] are true and [r] is the correct reason for [a]
- BBoth [a] and [r] are true but [r] is not the correct reason for [a]
- CBoth [a] and [r] are false
- D[a] is true but [r] is false
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Match the morphology in Group I with the corresponding microorganism in Group II
| Group I | Group II |
|---|---|
| P. Coccus | 1. Treponema |
| Q. Rod | 2. Bacillus |
| R. Comma | 3. Neisseria |
| S. Spiral | 4. Vibrio |
- AP – 3, Q – 2, R – 4, S – 1
- BP – 4, Q – 1, R – 3, S – 2
- CP – 2, Q – 4, R – 1, S – 3
- DP – 1, Q – 2, R – 3, S – 4
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Which one of the following genetic crosses and their results indicates cytoplasmic inheritance?
- AWild-type male × mutant female → 100% progeny are mutant
- BWild-type male × mutant female → 25% progeny are wild-type
- CMutant male × wild-type female → 50% progeny are mutant
- DMutant male × wild-type female → 75% progeny are wild-type
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Which of the following is NOT a characteristic morphological feature of apoptotic cells?
- ADisassembly of nuclear envelope
- BDNA fragmentation
- CIncreased cell size
- DMembrane blebbing
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Competition between two populations in an ecosystem is
- Abeneficial (+) to both the populations
- Bdeleterious (−) to both the populations
- Cbeneficial (+) to one population, but deleterious (−) to the other population
- Dbeneficial (+) to one population, but no effect (0) on the other population
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Adenine constitutes 0.16 mole fraction in a given single-stranded DNA. What is the mole fraction of uracil in the resultant RNA, if this entire DNA fragment is transcribed?
- A0.16
- B0.32
- C0.34
- D0.68
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Which one of the following is NOT used as a component in subunit vaccines?
- ACapsular polysaccharide
- BInactivated exotoxin
- CInactivated virus
- DViral glycoprotein
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Metabolic acidosis is associated with decreased plasma level of
- Abicarbonate
- Blactate
- Coxygen
- Durea
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Genes in two species that are derived from the same ancestral gene in their most recent common ancestor are called
- Aanalogs
- Bheterologs
- Corthologs
- Dparalogs
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An object is placed 15 cm in front of a convex mirror, which has a radius of curvature 30 cm. Which one of the following is true of the image formed?
- AReal and inverted
- BReal and upright
- CVirtual and inverted
- DVirtual and upright
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If a variable z shows a standard normal distribution, then the percent probability that 0 ≤ z2 ≤ 1 is ___________ (rounded off to the nearest integer).
- A34
- B68
- C95
- D99
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In chick embryo, the ectoderm generates
- Aalveolar cells
- Bgerm cells
- Cneurons
- Dred blood cells
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The boiling points of Iodomethane, Dibromomethane, Bromomethane, Chloromethane follow the order
- ABromomethane > Dibromomethane > Iodomethane > Chloromethane
- BBromomethane > Iodomethane > Chloromethane > Dibromomethane
- CDibromomethane > Iodomethane > Bromomethane > Chloromethane
- DIodomethane > Bromomethane > Chloromethane > Dibromomethane
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Chromosome duplication during the cell cycle occurs in
- AG1 phase
- BG2 phase
- CM phase
- DS phase
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Ionic character of the covalent bonds in the compounds Cl2, HCl, NaCl, NaF follows the order
- ACl2 > NaCl > HCl > NaF
- BHCl > Cl2 > NaF > NaCl
- CHCl > NaCl > NaF > Cl2
- DNaF > NaCl > HCl > Cl2
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Section B: Multiple select (one or more correct) (Q31–40)
10 questions, 2 marks each. No negative marking.
Which of the following is/are lateral meristems?
- ACork cambium
- BProcambium
- CProtoderm
- DVascular cambium
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Which of the following statement(s) about Golden Rice is/are correct?
- AConsumption of it increases vitamin A levels
- BConsumption of it increases vitamin D levels
- CConsumption of it increases vitamin K levels
- DIt is a transgenic crop containing β-carotene
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Which of the following statement(s) about eukaryotic DNA topoisomerase is/are correct?
- ATopoisomerase I creates transient single-strand breaks
- BTopoisomerase I creates transient double-strand breaks
- CTopoisomerase II creates transient single-strand breaks
- DTopoisomerase II creates transient double-strand breaks
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Which of the following method(s) is/are used to estimate protein concentration?
- AAnthrone
- BBiuret
- CBradford
- DLowry
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Which of the following is/are example(s) of a lotic ecosystem?
- ALake
- BPond
- CRiver
- DStream
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Which of the following statement(s) about the effect of genetic drift is/are correct?
- AIt can cause changes in the frequency of alleles at random
- BIt is a mechanism of evolution
- CIt can lead to loss of genetic variation within small populations
- DIt is significant in large populations
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Which of the following technique(s) can be used to determine the three-dimensional structure of an organic compound?
- AMass spectrometry
- BNMR spectroscopy
- CUV-visible spectroscopy
- DX-ray crystallography
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Which of the following entity(ies) is/are found inside the intact nucleus of eukaryotic cells?
- ACentrosome
- BLysosome
- CNucleolus
- DNucleosome
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Which of the following is/are trace element(s)?
- AMn
- BP
- CS
- DZn
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Which of the following is/are true about Retrovirus?
- AIt contains double-stranded RNA genome
- BIt can cause cancer
- CIt contains reverse transcriptase
- DIt contains double-stranded DNA genome
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Section C: Numerical answer (Q41–60)
20 questions (10 of 1 mark, 10 of 2 marks). No negative marking.
A wooden plant accumulates 10 mg kg−1 of 14C during its life span. A fossil of this plant was discovered and contains 2.5 mg kg−1 of 14C. The age of this fossil at the time of discovery is __________ years (rounded off to the nearest integer).
(Use 5730 years as half-life of 14C)
Type a number as your answer.
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A cylinder contains 50 L of an ideal gas at a pressure of 50 atm. Assuming that the temperature remains unchanged, the volume of the gas at 1 atm is __________ L (rounded off to the nearest integer).
Type a number as your answer.
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One molecule of the protein myoglobin contains one atom of iron. A myoglobin sample was found to contain 0.34% iron. The molecular weight of myoglobin is __________ g mol−1 (rounded off to the nearest integer).
(Use 55.9 g mol−1 as atomic mass of iron)
Type a number as your answer.
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The wavelength of visible light for the green color is 600 nm. The energy of photons of this color is __________ eV (rounded off to one decimal place).
(Planck’s constant = 6.63 × 10−34 J s, 1 eV = 1.6 × 10−19 J, speed of light = 3 × 108 m s−1)
Type a number as your answer.
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A ball dropped from a bridge hits the surface of the water in 3 s. The height of the bridge, ignoring air resistance, is __________ m (rounded off to one decimal place).
(Use g = 9.8 m s−2)
Type a number as your answer.
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For a given square, if the area of its incircle is 100 cm2, then the area of its circumcircle is __________ cm2 (rounded off to the nearest integer).
Type a number as your answer.
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The number of peaks in the 1H NMR spectrum of methoxymethane (CH3OCH3) is __________.
Type a number as your answer.
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The amount of agarose required to prepare 250 mL of 0.8% agarose gel is __________ grams (rounded off to the nearest integer).
Type a number as your answer.
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Three genes x, y, and z are located on a chromosome in a linear order. If the recombination frequencies between x and y is 0.15, and between y and z is 0.10, then the expected frequency of double crossovers is __________ (rounded off to three decimal places).
Type a number as your answer.
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A bacterial cell suspension contains 2 × 105 cells mL−1. The volume of this suspension required to obtain 1.4 × 106 cells is __________ mL (rounded off to the nearest integer).
Type a number as your answer.
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The data provided in the table were obtained from the following reaction, carried out at 273 K.
A + B → C
| Initial concentration of [A] mol L−1 | Initial concentration of [B] mol L−1 | Initial rate of formation of [C] mol L−1 s−1 |
|---|---|---|
| 0.2 | 0.2 | 0.3 |
| 0.4 | 0.2 | 0.6 |
| 0.4 | 0.4 | 2.4 |
The order of the reaction with respect to A is __________.
Type a number as your answer.
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Ammonia is synthesized in the Haber process in the following reaction.
N2(g) + 3H2(g) → 2NH3(g)
The temperature above which the reaction becomes spontaneous is __________ K (rounded off to one decimal place).
(ΔH0 = −92.2 kJ, ΔS0 = −199 J K−1)
Type a number as your answer.
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In the given molecule,

the number of chiral centers is __________.
Type a number as your answer.
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Two resistors 2 Ω and 4 Ω are combined in parallel. If this combination is connected to a battery of 16 V, the maximum current that can be drawn from the battery is __________ A (rounded off to the nearest integer).
Type a number as your answer.
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A box of mass 20 kg is pulled at constant speed across a floor by a rope. The rope makes an angle of 45° with the horizontal. Assuming that friction is negligible, the work done in pulling the box by a distance of 20 m is __________ J (rounded off to the nearest integer).
(Use g = 9.8 m s−2)
Type a number as your answer.
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Consider an enzyme that follows simple Michaelis-Menten kinetics, and has a KM of 5 μM. The initial velocity of the reaction will be 10% of the maximum velocity at a substrate concentration of __________ μM (rounded off to two decimal places).
Type a number as your answer.
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The value of limx→3 (x2 − 9) / (x2 − 4x + 3) is __________ (rounded off to the nearest integer).
Type a number as your answer.
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A population of 1000 plants are in Hardy-Weinberg equilibrium. Two alleles R and r determine a particular trait in this population. If the number of plants with RR genotype is 640, Rr genotype is 320 and rr genotype is 40, the frequency of r allele (in percentage) in this population is __________ (rounded off to the nearest integer).
Type a number as your answer.
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If a fair coin is tossed two times, the probability that the first or the second toss will be heads is __________ (rounded off to two decimal places).
Type a number as your answer.
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The restriction map of a circular plasmid is shown below, along with the indicated distances between the restriction sites.

The plasmid was completely digested with EcoRI, and XhoI. The products were analysed by agarose gel electrophoresis followed by ethidium bromide staining. The number of bands that will be visible in the gel when exposed to UV light is __________.
Type a number as your answer.
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Changes in the answer key
No question was dropped or given marks to all, and each question has the single answer shown.
This is IIT Madras’s provisional answer key (the final key is no longer online), so it may differ from the final key on a few questions.
How to revise from this paper
- Attempt it in one 3-hour sittingAll 60 questions are compulsory, as in the real exam. Check answers only at the end.
- Score it the JAM wayAdd 1 or 2 marks per correct answer and subtract 1/3 or 2/3 only for wrong MCQs. MSQ must be fully correct to score.
- Sort your mistakes by topicUse the topic tag on each question. Two or three misses in the same topic show where to revise first.
- Repeat with another yearTake a different year a week later and compare your topic-wise mistakes.

