
All 122 questions from the GATE 2024 Life Sciences (XL) paper, typed out in full, with the official final answer key and every diagram: General Aptitude, Chemistry and all five optional sections.
GATE 2024 Life Sciences (XL) was organised by IISc Bengaluru. Every candidate answered General Aptitude (GA) and XL-P Chemistry, then chose two of the five optional sections: XL-Q Biochemistry, XL-R Botany, XL-S Microbiology, XL-T Zoology and XL-U Food Technology. This page has all of them, in the order they were set, so you can practise the two you plan to choose and look at the rest. Each question is tagged with its topic. Tap Show answer under any question to check the official answer.
Marking scheme
| Section | Questions | Marks | You answer |
|---|---|---|---|
| GA: General Aptitude | 10 (5 × 1 mark, 5 × 2 marks) | 15 | Compulsory |
| XL-P: Chemistry | 17 (9 × 1 mark, 8 × 2 marks) | 25 | Compulsory |
| XL-Q to XL-U: five optional sections | 19 each (8 × 1 mark, 11 × 2 marks) | 30 each | Any two |
| Total you answer | 65 | 100 | 3 hours |
Which topics this paper tested
We tagged every question with its GATE XL syllabus topic. The table shows the three biggest topics in each section; each section below opens with a chart of all its topics.
| Section | Questions | Most-tested topics (questions) |
|---|---|---|
| GA General Aptitude | 10 | Quantitative Aptitude (6), Verbal Aptitude (2), Spatial Aptitude (1) |
| XL-P Chemistry | 17 | Organic Chemistry (5), Atomic Structure & Periodicity (2), Thermodynamics (2) |
| XL-Q Biochemistry | 19 | Biomolecules (5), Biochemical Techniques (4), Metabolism & Bioenergetics (3) |
| XL-R Botany | 19 | Plant Physiology & Biochemistry (5), Morphogenesis & Development (2), Plant Pathology (2) |
| XL-S Microbiology | 19 | Prokaryotic Cells (4), Methods in Microbiology (3), Microbial Genetics (3) |
| XL-T Zoology | 19 | Biochemistry & Molecular Biology (6), Parasitology & Immunology (3), Animal Anatomy & Physiology (3) |
| XL-U Food Technology | 19 | Food Chemistry & Nutrition (7), Food Microbiology (5), Food Products Technology (4) |
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GA: General Aptitude (Q1–10)
10 questions (5 of 1 mark, 5 of 2 marks). Compulsory for every XL candidate.
If ‘→’ denotes increasing order of intensity, then the meaning of the words [walk → jog → sprint] is analogous to [bothered → ________ → daunted]. Which one of the given options is appropriate to fill the blank?
- Aphased
- Bphrased
- Cfazed
- Dfused
Show answer
Two wizards try to create a spell using all the four elements, water, air, fire, and earth. For this, they decide to mix all these elements in all possible orders. They also decide to work independently. After trying all possible combination of elements, they conclude that the spell does not work. How many attempts does each wizard make before coming to this conclusion, independently?
- A24
- B48
- C16
- D12
Show answer
In an engineering college of 10,000 students, 1,500 like neither their core branches nor other branches. The number of students who like their core branches is 1/4th of the number of students who like other branches. The number of students who like both their core and other branches is 500.
The number of students who like their core branches is
- A1,800
- B3,500
- C1,600
- D1,500
Show answer
For positive non-zero real variables x and y, if
ln((x + y)/2) = ½[ln(x) + ln(y)]
then, the value of x/y + y/x is
- A1
- B1/2
- C2
- D4
Show answer
In the sequence 6, 9, 14, x, 30, 41, a possible value of x is
- A25
- B21
- C18
- D20
Show answer
Sequence the following sentences in a coherent passage.
P: This fortuitous geological event generated a colossal amount of energy and heat that resulted in the rocks rising to an average height of 4 km across the contact zone.
Q: Thus, the geophysicists tend to think of the Himalayas as an active geological event rather than as a static geological feature.
R: The natural process of the cooling of this massive edifice absorbed large quantities of atmospheric carbon dioxide, altering the earth’s atmosphere and making it better suited for life.
S: Many millennia ago, a breakaway chunk of bedrock from the Antarctic Plate collided with the massive Eurasian Plate.
- AQPSR
- BQSPR
- CSPRQ
- DSRPQ
Show answer
A person sold two different items at the same price. He made 10% profit in one item, and 10% loss in the other item. In selling these two items, the person made a total of
- A1% profit
- B2% profit
- C1% loss
- D2% loss
Show answer
The pie charts depict the shares of various power generation technologies in the total electricity generation of a country for the years 2007 and 2023.

The renewable sources of electricity generation consist of Hydro, Solar and Wind. Assuming that the total electricity generated remains the same from 2007 to 2023, what is the percentage increase in the share of the renewable sources of electricity generation over this period?
- A25%
- B50%
- C77.5%
- D62.5%
Show answer
A cube is to be cut into 8 pieces of equal size and shape. Here, each cut should be straight and it should not stop till it reaches the other end of the cube. The minimum number of such cuts required is
- A3
- B4
- C7
- D8
Show answer
In the 4 × 4 array shown below, each cell of the first three rows has either a cross (X) or a number.

The number in a cell represents the count of the immediate neighboring cells (left, right, top, bottom, diagonals) NOT having a cross (X). Given that the last row has no crosses (X), the sum of the four numbers to be filled in the last row is
- A11
- B10
- C12
- D9
Show answer
XL-P: Chemistry (Q11–27)
17 questions (9 of 1 mark, 8 of 2 marks). Compulsory for every XL candidate.
The CORRECT order of electronegativity is
- AAl > Si > P > S
- BAl > S > Si > P
- CS > Si > Al > P
- DS > P > Si > Al
Show answer
Which one of the following is the CORRECT representation of the variation of the Gibbs free energy (G) of a substance with temperature (T) at constant pressure?
- A

- B

- C

- D

Show answer
Among the following, the structure representing histidine is
- A

- B

- C

- D

Show answer
The CORRECT order of acidity of the following compounds is

- AI > II > III
- BII > III > I
- CI > III > II
- DIII > II > I
Show answer
The molecules A and B are a pair of ____________.

- Aenantiomers
- Bdiastereomers
- Cconformational isomers
- Dconstitutional isomers
Show answer
The CORRECT option(s) of Y for the following reaction is/are

- A

- B

- C

- D

Show answer
The maximum number of electrons that can be accommodated in the shell with n = 2 is ________ (in integer). (Given: n = principal quantum number)
Type a number as your answer.
Show answer
One mole of an ideal gas expands isothermally and reversibly to double its volume. If the expansion work done by the system is 1728.85 J, the temperature of the system is _____ K (rounded off to 2 decimal places). (Given: Gas constant, R = 8.314 J K–1 mol–1)
Type a number as your answer.
Show answer
The initial rate of a reaction triples when the concentration of a reactant, A, is doubled. The order of the reaction with respect to A is _______ (rounded off to 2 decimal places).
Type a number as your answer.
Show answer
Each of the following alkenes undergoes addition reaction with bromine. Under the same reaction conditions, the CORRECT trend in the reaction rates is

- AI > II > III
- BII > III > I
- CI > III > II
- DIII > II > I
Show answer
An enzyme-catalyzed conversion of a substrate at 298 K proceeds by a Michaelis-Menten mechanism. The Lineweaver-Burk plot for the analysis of the experimental data has an intercept along the y-axis of 0.357 mmol–1 dm3 s and a slope of 2.10 s. The CORRECT Michaelis constant for the reaction is ________ (rounded off to 2 decimal places).
- A5.88 mmol dm–3
- B5.88 mmol dm–3 s–1
- C2.80 mmol dm–3
- D2.80 mmol dm–3 s–1
Show answer
Which one among the following structures is the most stable conformer of (Z)-pent-2-ene?
- A

- B

- C

- D

Show answer
Upon addition of compound X to an aqueous AgNO3 solution, a white precipitate appears instantly. Also, X does not exhibit geometrical isomerism.
The CORRECT option(s) for X is/are
- A[Cr(OH2)4Cl2]Cl
- B[Cr(OH2)5Cl]Cl2
- C[Cr(OH2)6]Cl3
- D[Cr(OH2)3Cl3]
Show answer
The paramagnetic species among the following is/are
(Given: Atomic numbers of Cr = 24; Fe = 26; Ni = 28)
- A[Fe(CN)6]3–
- B[Ni(OH2)6]2+
- C[Ni(CN)4]2–
- D[Cr(CN)6]3–
Show answer
The molecule(s) with non-zero dipole moment is/are
- AN2
- BCO2
- CNO
- DSO2
Show answer
The ionic product of water at 40 ℃ is 2.92 × 10–14 M2. The pH of water at 40 ℃ is ___________ (rounded off to 2 decimal places).
Type a number as your answer.
Show answer
Given the standard reduction potentials (E⊖) for the half-cell reactions below, the standard Gibbs free energy of the dissolution of silver chloride in water, at 298 K, is _____________ J mol–1 (rounded off to nearest integer).
(Given: Faraday constant, F = 96500 C mol–1; J = C × V)
AgCl(s) + e− → Ag(s) + Cl−(aq) ; E⊖ = 0.22 V at 298 K
Ag+(aq) + e− → Ag(s) ; E⊖ = 0.80 V at 298 K
Type a number as your answer.
Show answer
XL-Q: Biochemistry (Q28–46)
19 questions (8 of 1 mark, 11 of 2 marks). Optional: answer it only if you pick Biochemistry. From GATE 2027 this section is called XL1.
Which one of the following pairs of amino acids is NOT incorporated in a polypeptide chain?
- A4-Hydroxyproline and γ-carboxyglutamate
- Bγ-Carboxyglutamate and desmosine
- COrnithine and citrulline
- D4-Hydroxyproline and 5-hydroxylysine
Show answer
Mammalian cells cultured at low temperature (25 to 30 °C) leads to an increased sterol content in the membrane. Elevated sterols in the membrane results in
- Aan enhancement in membrane fluidity.
- Bstabilization of membrane proteins.
- Can increase in membrane permeability to water.
- Da decrease in membrane fluidity.
Show answer
Which one of the following metabolic intermediates is common to glycolysis, nucleotide synthesis and glycogen synthesis?
- ACitrate
- BOxaloacetate
- CGlucose 6-phosphate
- DGlycerol 3-phosphate
Show answer
In mammals, hematopoietic stem cells that give rise to different types of blood cells are known as
- Atotipotent stem cells.
- Bpluripotent stem cells.
- Cmyeloid progenitor cells.
- Dlymphoid progenitor cells.
Show answer
Which one or more of the following statements correctly describe(s) the addition of N-nucleotides during the rearrangement of the immunoglobulin heavy chain-encoding gene?
- AAddition of N-nucleotides is template encoded.
- BN-nucleotides are added by terminal deoxynucleotidyl transferase.
- CThe added N-nucleotides are common in V-D and D-J junction.
- DN-nucleotides are added by the DNA polymerase II.
Show answer
A newly identified viral protein contains one long α-helix spanning 60 amino acid residues. The number of main chain H-bonds formed in this helix is __________. (Answer in integer)
Type a number as your answer.
Show answer
In a lactic acid solution at pH 4.8, the concentrations of lactic acid and lactate are 0.01 M and 0.087 M, respectively. The calculated pKa of lactic acid is ________. (Round off to one decimal place)
Type a number as your answer.
Show answer
If a 10 mM solution of a biomolecule in a cuvette of path length 10 mm absorbs 90% of the incident light at 280 nm, the molar extinction coefficient of the biomolecule at this wavelength is _____________ M-1cm-1. (Round off to two decimal places)
Type a number as your answer.
Show answer
Metabolic intermediates provide the backbone for the synthesis of amino acids. Match the metabolic intermediates listed in Column I with their corresponding amino acids given in Column II.
| Column I | Column II |
|---|---|
| P. α-Ketoglutarate | i. Histidine |
| Q. Ribose 5-phosphate | ii. Glutamate |
| R. 3-Phosphoglycerate | iii. Aspartate |
| S. Phosphoenolpyruvate | iv. Phenylalanine |
| v. Serine |
- AP-ii; Q-i; R-v; S-iv
- BP-iii; Q-ii; R-i; S-v
- CP-iv; Q-iii; R-ii; S-v
- DP-ii; Q-i; R-iv; S-v
Show answer
Which one of the following is the correct match between the molecular properties listed in Column I and the corresponding biochemical separation methods in Column II?
| Column I | Column II |
|---|---|
| P. Solubility | i. Reverse phase chromatography |
| Q. Ionic charge | ii. Ultracentrifugation |
| R. Polarity | iii. Salting out |
| S. Molecular size | iv. Isoelectric focusing |
| v. Gel electrophoresis |
- AP-i; Q-ii; R-v; S-iii
- BP-iii; Q-iv; R-ii; S-i
- CP-iii; Q-iv; R-i; S-ii
- DP-v; Q-iv; R-iii; S-ii
Show answer
Which one or more of the following statements is/are correct regarding the electromotive force generated by electron transfer chain?
- AIt is used for the synthesis of ATP.
- BIt is not used for active transport process.
- CIt includes a pH gradient component.
- DIt does not include an electrical potential gradient component.
Show answer
Which one or more of the following statements is/are correct regarding the transport and retention of proteins in different cell organelles?
- AMannose 6-phosphate residues are involved in targeting proteins to lysosomes.
- BTransport of proteins into the mitochondrial compartment is aided by positively charged amino acid residues at the N-terminus and internal hydrophobic segments.
- CThe retention of protein in the ER lumen requires the KDEL sequence motif at the C-terminus.
- DNuclear proteins are transported in an unfolded conformation and the nuclear localization signal sequence is subsequently cleaved by peptidases in the nucleoplasm.
Show answer
Which one or more of the following statements correctly describe(s) fluorescence spectroscopy?
- AThe emission maxima (λmax) is independent of the excitation wavelength.
- BThe emission maxima (λmax) depends on the concentration of a quencher.
- CThe emission maxima (λmax) varies with solvent polarity.
- DThe emission maxima (λmax) varies with temperature.
Show answer
Which one or more of the following statements is/are correct in the processing of pre-mRNA in eukaryotes?
- A3′ → 5′ exonuclease activity is involved in the conversion of pre-mRNA to mRNA.
- B5′– capping and addition of 3′– poly A tail precedes splicing.
- CSplicing of pre-mRNA occurs via transesterification reaction.
- DAlternative splicing can yield different mRNA products from the same pre-mRNA.
Show answer
Which one or more of the following statements correctly describe(s) the changes upon the addition of puromycin during eukaryotic translation?
- APuromycin resembles aminoacyl end of the charged tRNA.
- BPuromycin occupies the A site of the translating ribosomes.
- CPuromycin occupies the P site of the translating ribosomes.
- DPuromycin occupies the E site of the translating ribosomes.
Show answer
Factor H, a complement regulatory protein in plasma, binds C3b and
- Acompetes with factor B to displace Bb from convertase.
- Binitiates the catabolism of C3b into inactivate products.
- Cthen binds to C3bBb convertase.
- Dacts as a cofactor for factor I.
Show answer
In Michaelis-Menten’s equation, if [S] = 15 Km, then the ratio v0/Vmax is __________. (Round off to three decimal places)
Type a number as your answer.
Show answer
A 5250 base-pair long plasmid with 10 negative supercoils would have a linking number of ____________, considering 10.5 base pairs per turn for B DNA. (Answer in integer)
Type a number as your answer.
Show answer
The spectrum of a protein obtained using electrospray ionization mass spectrometry (ESI-MS) is shown below. Two peaks, one at m/z = 2960.6 and the other at m/z = 3552.5, are marked. The mass of the protein associated with the m/z = 2960.6 peak is __________ Da. (Round off to two decimal places)

Type a number as your answer.
Show answer
XL-R: Botany (Q47–65)
19 questions (8 of 1 mark, 11 of 2 marks). Optional: answer it only if you pick Botany. From GATE 2027 this section is called XL2.
Which one of the following plant families does apple (Malus domestica) belong to?
- ARosaceae
- BRutaceae
- CRubiaceae
- DRanunculaceae
Show answer
The collateral and open type of vascular bundle with endarch xylem strand is usually found in
- Amonocot stem
- Bdicot stem
- Cmonocot root
- Ddicot root
Show answer
Which of the following tissue types is/are established during embryogenesis in wild-type Arabidopsis thaliana?
- AShoot apical meristem
- BRosette leaf primordium
- CProcambium
- DLateral root primordium
Show answer
Which of the following plant natural products is/are cyanogenic glycoside(s)?
- ALinustatin
- BLimonene
- CLuteolin
- DLinamarin
Show answer
Which of the following plant diseases is/are caused by nematode?
- ACereal cyst of barley
- BErgot of rye
- CWart of potato
- DEar-cockle of wheat
Show answer
Which of the following selectable marker genes is/are used for herbicide tolerance during genetic transformation of plants?
- Ahpt
- Bbar
- CnptII
- Dpmi
Show answer
Which of the following statements is/are CORRECT with reference to rubber production from plants?
- APara rubber is produced from Hevea brasiliensis
- BIndia rubber is produced from Ficus elastica
- CPanama rubber is produced from Manihot glaziovii
- DCeara rubber is produced from Castilla elastica
Show answer
In Calvin-Benson cycle, to produce 1 molecule of glyceraldehyde 3-phosphate by fixing 3 molecules of carbon dioxide, 9 molecules of ATP and ________ molecules (in integer) of NADPH are typically utilized.
Type a number as your answer.
Show answer
In wild-type Arabidopsis thaliana, the four types of floral organs (sepal, petal, stamen, carpel) are arranged in concentric whorls from outside to inside. With reference to the ABC model of floral organ patterning, match the homeotic mutants in Group 1 with their respective arrangements of organs in the four whorls given in Group 2.
| Group 1 | Group 2 |
|---|---|
| (P) A class mutants | (i) sepal, sepal, carpel, carpel |
| (Q) B class mutants | (ii) sepal, petal, petal, sepal |
| (R) C class mutants | (iii) carpel, stamen, stamen, carpel |
| (iv) sepal, sepal, petal, petal |
- AP–iv, Q–ii, R–i
- BP–iii, Q–i, R–ii
- CP–ii, Q–i, R–iii
- DP–iii, Q–i, R–iv
Show answer
Match the inhibitors in Group 1 with their respective targets in Group 2.
| Group 1 | Group 2 |
|---|---|
| (P) Oligomycin | (i) Cytochrome bc1 complex |
| (Q) Antimycin A | (ii) Photosystem II |
| (R) 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) | (iii) K+ ionophore |
| (S) Valinomycin | (iv) FO ATP synthase |
- AP–i, Q–ii, R–iii, S–iv
- BP–iv, Q–i, R–ii, S–iii
- CP–iii, Q–i, R–iv, S–ii
- DP–iv, Q–ii, R–i, S–iii
Show answer
With reference to Agrobacterium tumefaciens mediated plant transformation, match the virulence factors in Group 1 with their protein types in Group 2.
| Group 1 | Group 2 |
|---|---|
| (P) VirG | (i) Kinase |
| (Q) VirA | (ii) Helicase |
| (R) VirE | (iii) Transcriptional activator |
| (S) VirC | (iv) Single strand binding protein |
- AP–i, Q–ii, R–iv, S–iii
- BP–iii, Q–i, R–ii, S–iv
- CP–ii, Q–iv, R–i, S–iii
- DP–iii, Q–i, R–iv, S–ii
Show answer
Match the plant products in Group 1 with the plant species in Group 2 that produce them and the respective plant parts in Group 3 where they accumulate the most.
| Group 1 | Group 2 | Group 3 |
|---|---|---|
| (P) Liquorice | (i) Cinchona calisaya | (a) Leaf |
| (Q) Quinine | (ii) Lawsonia inermis | (b) Root |
| (R) Henna | (iii) Glycyrriza glabra | (c) Flower |
| (S) Saffron | (iv) Papaver somniferum | (d) Bark |
| (v) Crocus sativus | (e) Seed |
- AP–iii–b, Q–i–d, R–ii–a, S–v–c
- BP–i–b, Q–iii–d, R–ii–a, S–iv–c
- CP–iii–b, Q–i–d, R–ii–e, S–v–c
- DP–iv–b, Q–i–d, R–iii–c, S–ii–a
Show answer
Match the types of ecological interactions in Group 1 with their respective definitions in Group 2.
| Group 1 | Group 2 |
|---|---|
| (P) Protocooperation | (i) One species is harmed but the other is neither harmed nor benefitted |
| (Q) Commensalism | (ii) A type of mutualism where one species is benefitted more than the other |
| (R) Amensalism | (iii) Both the species are benefitted but the interaction between them is not obligatory |
| (S) Helotism | (iv) One species is benefitted without harming the other |
- AP–iii, Q–iv, R–i, S–ii
- BP–ii, Q–i, R–iii, S–iv
- CP–i, Q–iv, R–iii, S–ii
- DP–iii, Q–i, R–iv, S–ii
Show answer
Match the types of ecological energy productivity in Group 1 with their respective definitions in Group 2.
| Group 1 | Group 2 |
|---|---|
| (P) Net primary productivity | (i) Total amount of energy produced by autotrophs |
| (Q) Gross primary productivity | (ii) Amount of energy stored by autotrophs after respiration |
| (R) Net productivity | (iii) Net gain of energy by the consumers after energy loss |
| (S) Secondary productivity | (iv) Unused amount of energy after consumption by heterotrophs |
- AP–iii, Q–ii, R–iv, S–i
- BP–ii, Q–i, R–iii, S–iv
- CP–ii, Q–i, R–iv, S–iii
- DP–iv, Q–i, R–ii, S–iii
Show answer
Which of the following combinations of plant diseases and the types of their causal organisms is/are CORRECT?
- ALate blight of potato – Bacteria
- BBlack rot of crucifer – Bacteria
- CTungro disease of rice – Mycoplasma
- DRoot knot of tomato – Nematode
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Identify the CORRECT combination(s) of plant natural products and the categories they belong to.
- ADhurrin – Phenolic compounds
- BFarnesene – Terpenoids
- CNaringenin – Cyanogenic glycosides
- DVincristine – Alkaloids
Show answer
Identify the CORRECT combination(s) between the enzymes in Group 1 and the reactions in Group 2 they catalyze.
| Group 1 | Group 2 |
|---|---|
| (P) Cinnamate-4-hydroxylase | (i) L-phenylalanine → Cinnamic acid |
| (Q) Glycerate kinase | (ii) Glyceraldehyde 3-phosphate → dihydroxyacetone phosphate |
| (R) PEP carboxylase | (iii) Glycolate + O2 → Glyoxylate + H2O2 |
| (S) Nitrate reductase | (iv) NO3− + NAD(P)H + H+ → NO2− + NAD(P)+ + H2O |
- AS–iv
- BQ–ii
- CP–i
- DR–iii
Show answer
In a genetic cross between two pure-line parents differing in the two independently segregating traits, plant height (tall vs dwarf) and flower color (purple vs white), all the F1 plants were tall with purple flowers. In a testcross population involving these F1 individuals, the expected percentage (%) of dwarf plants with purple flower would be _______ (in integer).
Type a number as your answer.
Show answer
The mRNA of a hypothetical plant gene HSDU is 800-nucleotide long and encodes a protein of 160 amino acid residues. The calculated length of HSDU CDS would be ________ nucleotides (in integer).
Type a number as your answer.
Show answer
XL-S: Microbiology (Q66–84)
19 questions (8 of 1 mark, 11 of 2 marks). Optional: answer it only if you pick Microbiology. From GATE 2027 this section is called XL3.
Which one of the following bacterial species can cause atypical pneumonia?
- AChlamydia pneumoniae
- BStreptococcus pneumoniae
- CKlebsiella pneumoniae
- DHaemophilus influenzae
Show answer
Which one of the following organisms has axial filaments?
- AMycobacterium tuberculosis
- BPasteurella multocida
- CTreponema pallidum
- DShigella dysenteriae
Show answer
Who among the following scientists was the pioneer in development of chemotherapy?
- AElie Metchnikoff
- BRobert Koch
- CPaul Ehrlich
- DRonald Ross
Show answer
In which of following processes, glutaraldehyde is used as a sterilizing agent?
- APasteurization
- BIncineration
- CCold sterilization
- DAutoclaving
Show answer
The most abundant class of immunoglobulins in serum is __________.
- AIgM
- BIgA
- CIgD
- DIgG
Show answer
Which one of the following double-stranded sequences will NOT be recognized by a Type IIP restriction endonuclease?
- A5′–GGTACC–3′
3′–CCTAGG–5′ - B5′–GGATCC–3′
3′–CCTAGG–5′ - C5′–CATATG–3′
3′–GTATAC–5′ - D5′–GATTTC–3′
3′–CTAAAG–5′
Show answer
3′–CTAAAG–5′
Which one of the following use inorganic compounds as an energy source?
- AHeterotrophs
- BChemolithotrophs
- CChemoorganotrophs
- DPhotoheterotrophs
Show answer
Which one of the following represents the abundance of the organisms found in soil?
- AFungi > Aerobic bacteria > Anaerobic bacteria
- BAerobic bacteria > Fungi > Anaerobic bacteria
- CAerobic bacteria > Anaerobic bacteria > Fungi
- DAnaerobic bacteria > Aerobic bacteria > Fungi
Show answer
Match the antibiotics in Group I with the microorganisms that produce them in Group II.
| Group I | Group II |
|---|---|
| (P) Streptomycin | (i) Streptomyces griseus |
| (Q) Bacitracin | (ii) Bacillus licheniformis |
| (R) Amphotericin B | (iii) Streptomyces venezuelae |
| (S) Chloramphenicol | (iv) Streptomyces nodosus |
- A(P)-(ii), (Q)-(iii), (R)-(i), (S)-(iv)
- B(P)-(i), (Q)-(ii), (R)-(iv), (S)-(iii)
- C(P)-(i), (Q)-(ii), (R)-(iii), (S)-(iv)
- D(P)-(ii), (Q)-(iv), (R)-(i), (S)-(iii)
Show answer
Which one of the following redox couples has the highest tendency to donate electrons?
- AFumarate / succinate
- BNAD+ / NADH
- CFAD / FADH
- DPyruvate / lactate
Show answer
Which of the following is/are active transport mechanism(s) in prokaryotes where the substance is chemically altered during transport across the membrane?
- AGroup translocation
- BSimple diffusion
- CFacilitated diffusion
- DOsmosis
Show answer
Which of the following cocci is/are examples of division in one plane?
- AStaphylococci
- BStreptococci
- CMicrococci
- DDiplococci
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Which of the following event(s) occur(s) during translation in prokaryotes?
- AtRNA binding to the start codon of mRNA on the 30s subunit of ribosome
- BAnticodon of tRNA binding to the start codon of mRNA on the 50s subunit of ribosome
- CThe ribosome continues to move along the mRNA to add new amino acids to the polypeptide
- DThe polypeptide is released when the ribosome reaches the stop codon
Show answer
Which of the following is/are consequence(s) of nitrous acid (HNO2) mediated deamination?
- ADeamination of cytosine, adenine and guanine
- BGC-to-AT transitions
- CAT- to- GC transitions
- DAddition of alkyl group to the bases
Show answer
At root nodules, which of the following C4 organic acid(s) is/are transported across the symbiosome membrane and into bacteroids?
- ASuccinate
- BPyruvate
- CMalate
- DFumarate
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Which of the following is/are TRUE about the Escherichia coli chromosome?
- AIt is typically bound by histones
- BIt is circular in nature
- CIt is found in the nucleoid
- DIt contains multiple origins of replication
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At t = 0, the bacterial cell number is 10,000 cells/mL. At t = 480 minutes, the cell number increased to 320,000 cells/mL. The mean generation time during this exponential growth period, rounded off to the nearest integer is ________ minutes.
Type a number as your answer.
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A landfill sample was analyzed by dilution and plating techniques for viable bacterial count. When one gram of the landfill sample was diluted 1 x 10-4 (w/v) it yielded 400 CFU. The viable bacterial count (in million, rounded off to the nearest integer) in one gram landfill sample is _______.
Type a number as your answer.
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A fluorescence microscope with an objective lens of numerical aperture (NA) 1.5 is used with light of wavelength (λ) 600 nanometers. The lateral resolution limit of this microscope rounded off to the nearest integer, is _________ nanometers.
Type a number as your answer.
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XL-T: Zoology (Q85–103)
19 questions (8 of 1 mark, 11 of 2 marks). Optional: answer it only if you pick Zoology. From GATE 2027 this section is called XL4.
Which one of the following statements about gene expression is INCORRECT?
- ADNA is transcribed to mRNA.
- BmRNA can be reverse-transcribed to DNA.
- CmRNA can be translated to protein.
- DProtein can be reverse-translated to mRNA.
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Which one of the following tissues/organs is least likely to experience graft rejection when transplanted from a person to an unrelated person?
- Abone marrow
- Bcornea
- Cheart
- Dkidney
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Codon bias is correlated with the relative frequencies of which one of the following types of RNA?
- AmRNA
- BrRNA
- CsiRNA
- DtRNA
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CREB1 is a eukaryotic transcription factor. In which one of the following compartments of the cell is CREB1 predominantly localized?
- Alysosomes
- Bmitochondria
- Cnucleus
- Dperoxisomes
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In certain species of salamanders, male-female pairs have multiple mating partners in a breeding season. Which one of the following terminologies accurately describes this mating system?
- Amonogamy
- Bpolyandry
- Cpolygyny
- Dpolygynandry
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Which one of the following statements describes the key function of human sweat glands?
- AThey serve as touch sensors.
- BThey are responsible for skin color.
- CThey regulate body temperature.
- DThey store fat.
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Urease enzyme catalyzes the conversion of urea into ammonia and carbon dioxide. Which one of the following organisms expresses urease enzyme?
- ACaenorhabditis elegans
- BDrosophila melanogaster
- CHelicobacter pylori
- DHomo sapiens
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The human genetic code is triplet in nature with 64 codons made using four nucleotides. If the human genetic code was doublet in nature, the number of codons theoretically possible from four nucleotides is ______. (Answer in integer)
Type a number as your answer.
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Which one of the following statements is NOT TRUE of glycosaminoglycans?
- AGlycosaminoglycans are composed of repeating disaccharide units.
- BGlycosaminoglycans consist of amino sugars that are frequently sulfated.
- CHyaluronic acid is an example of a glycosaminoglycan.
- DMethionine is the predominant amino acid to which glycosaminoglycan chains are conjugated to form proteoglycans.
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Which one of the options correctly matches the human tissues/organs with their embryonic germ layers of origin?
| Tissues/organs | Embryonic germ layers |
|---|---|
| (P) liver | (I) ectoderm |
| (Q) cerebellum | (II) endoderm |
| (R) femur | (III) mesoderm |
- AP-II, Q-I, R-III
- BP-III, Q-I, R-II
- CP-I, Q-II, R-III
- DP-II, Q-III, R-I
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Consider a large population of a finch species, where both small and big beak sizes are advantageous, and an intermediate beak size is maladaptive. Over a period of 10 years, which one of the following evolutionary processes is most likely to operate on the beak size of this finch population?
- Adirectional selection
- Bdisruptive selection
- Cgenetic drift
- Dstabilizing selection
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When the blood glucose level of a healthy person is 100 mg/dL, which one of the following options is most likely to represent the level of glucose in the urine of that person?
- A< 1 mg/dL
- B10 mg/dL
- C50 mg/dL
- D100 mg/dL
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Which one of the following rooted tree topologies best describes the primate phylogeny?

- AI
- BII
- CIII
- DIV
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Consider a species of brightly colored beetle. Which one or more of the following observations suggest(s) that this species is aposematic?
- ABoth male and female beetles are brightly colored.
- BOnly male beetles are brightly colored.
- COnly female beetles are brightly colored.
- DThe beetle species is toxic and distasteful.
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The embryos of which one or more of the following animals show meroblastic cleavage?
- ADanio rerio (zebrafish)
- BGallus gallus (chicken)
- CSynapta digita (sea cucumber)
- DXenopus laevis (frog)
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Which one or more of the following parasites is/are typically transmitted by mosquitoes as vector?
- ALeishmania donovani
- BPlasmodium vivax
- CWuchereria bancrofti
- DTrichuris trichiura
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Consider the following nucleotide sequence:
5′-GCCGCCAUGGCGUCUGCUAGCUGGCUCGAUCGCGAGCGAUCGUACGUAUAGUAUGAA-3′
Assume canonical initiation, canonical termination, no post-translational modification, and the average molecular mass of an amino acid to be 110 daltons. The theoretical molecular mass of the polypeptide translated from the above nucleotide sequence is _____ daltons. (Answer in integer)
Type a number as your answer.
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The pKa of a buffer solution with pH of 5, consisting of 0.4 M sodium acetate and 0.04 M acetic acid, is ______. (Answer in integer)
Type a number as your answer.
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Consider a healthy person with the following lung volumes:
Residual volume = 900 mL
Expiratory reserve volume = 800 mL
Tidal volume = 200 mL
If the Total lung capacity is 5500 mL, then the Inspiratory reserve volume of the person is _____ mL. (Answer in integer)
Type a number as your answer.
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XL-U: Food Technology (Q104–122)
19 questions (8 of 1 mark, 11 of 2 marks). Optional: answer it only if you pick Food Technology. From GATE 2027 this section is called XL5.
Which one of the following fungi produces aflatoxins?
- AAspergillus niger
- BFusarium verticillioides
- CAspergillus flavus
- DRhizopus oligosporus
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Under standard conditions in animal feeding studies, the weight gained (in grams) per gram of protein consumed by an animal is termed as
- ANet Protein Ratio
- BNet Protein Utilization
- CCoefficient of Protein Digestibility
- DProtein Efficiency Ratio
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Xeropthalmia is caused due to the deficiency of
- AThiamin
- BPantothenic acid
- CVitamin A
- DVitamin C
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Which one of the following steps is used to remove phosphatides from crude oil in the refining process?
- ANeutralization
- BBleaching
- CDegumming
- DDeodorization
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The unique flavor of chocolate and cocoa is due to the formation of
- A5-methyl-2-phenyl-2-hexenal
- BCyclotene
- CFuraneol
- DMaltol
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Which one of the following statements regarding Hazard Analysis Critical Control Point (HACCP) plan is NOT correct?
- AHACCP is a management tool for ensuring food safety.
- BHACCP involves five preliminary steps and seven principles.
- CHACCP is not effective without prior implementation of prerequisite programs.
- DHACCP plan involves establishment of corrective actions as second principle.
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The product of cabbage fermentation by Leuconostoc mesenteroides is
- ATempeh
- BNatto
- CSauerkraut
- DMiso
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Which one of the following absorbents is NOT used as an ethylene absorber in active packaging of fruits and vegetables?
- APotassium permanganate
- BActivated carbon
- CCalcium hydroxide
- DSilica gel
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Which one of the following statements regarding moisture sorption isotherms of a dried food is NOT correct?
- AAt a given temperature, the difference between adsorption and desorption moisture isotherms is known as hysteresis.
- BAt a given temperature and water activity, an adsorption isotherm exhibits higher equilibrium moisture content than a desorption isotherm in hysteresis.
- CAt a given moisture content, effect of temperature on a moisture sorption isotherm follows the Clausius- Clapeyron equation.
- DThe Guggenheim-Anderson-de Boer (GAB) equation is a multilayer moisture sorption model.
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Processing of fluid milk at 72 ℃ for 15 seconds is termed as
- AHigh-temperature, short-time (HTST) pasteurization
- BLow-temperature, long-time (LTLT) pasteurization
- CUltra high-temperature (UHT) pasteurization
- DHomogenization process
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Match the anti-nutritional factor in Column I with their corresponding activity given in Column II.
| Column I | Column II |
|---|---|
| P. Lectin | 1. Flatulence |
| Q. Stachyose | 2. Chelates with divalent cations and reduces their bio-availability |
| R. Phytate | 3. Inhibits trypsin and chymotrypsin |
| S. Knuitz type inhibitor | 4. Hemagglutination |
- AP-4,Q-1, R-2,S-3
- BP-3,Q-1, R-2,S-4
- CP-2,Q-1, R-4,S-3
- DP-1,Q-2, R-3,S-4
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Which of the following fatty acids is/are known to increase the low density lipoprotein (LDL)-cholesterol?
- AOmega-3 Fatty acids
- BTrans Fatty acids
- CConjugated Linoleic acids
- DSaturated Fatty acids
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The addition of which of the following to high-methoxyl pectin will result in gel formation?
- ACalcium ions
- BHydrogen ions
- CSodium ions
- DSugar
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Which of the following steps in food processing is/are used to reduce the acrylamide formation in food products?
- APretreatment using asparaginase
- BLowering the pH
- CIncreasing the temperature.
- DAdding glucose.
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Which of the following enzymes is/are used for the production of high fructose syrup (HFS) from corn starch?
- Aα-Amylase
- Bβ-Amylase
- CXylose isomerase
- DGlucoamylase
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Which of the following is/are typical characteristic(s) of a fungal cell?
- APresence of histone proteins
- BPresence of peptidoglycans in the cell wall
- CPresence of chitin in the cell wall
- DPresence of pseudomurein in the cell wall
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Which of the following statements is/ are correct regarding food and water borne disease and the class of causative microorganisms?
- ALegionellosis is a bacterial disease.
- BGiardiasis is caused by the protists.
- CTyphoid fever is caused by the virus.
- DListeriosis is a fungal disease.
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Which of the following statements is/ are true?
- AHagen-Poiseuille’s law is used for calculation of molecular diffusion.
- BFick’s law is used for calculation of energy requirement in size reduction.
- CRittinger’s law is used for calculation of energy requirement in size reduction.
- DStokes law is used for derivation of terminal velocity.
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A 10 kg tomato pulp is concentrated from an initial moisture content of 90% (wet weight basis) to 35% (wet weight basis). The weight of the concentrate in kg is __________ (round off to 2 decimal places).
Type a number as your answer.
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Changes in the final answer key
No question was dropped or given marks to all in the final key, so each question below has the answer shown.
How to revise from this paper
- Pick your two optional sections firstAttempt GA, XL-P and the two sections you plan to choose, 65 questions in one 3-hour sitting.
- Score it the GATE wayAdd 1 or 2 marks per correct answer and subtract 1/3 or 2/3 only for wrong MCQs. MSQ must be fully correct to score.
- Sort your mistakes by topicUse the topic tag on each question. Two or three misses in the same topic show where to revise first.
- Repeat with another yearTake a different year a week later and compare your topic-wise mistakes.

















